IGEM:Groningen/Notebook/iGEM 2011/2011/05/31

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31-5-11 Transformation of overnight ligation Add to 40μl competent cells 10μl ligation mixture Incubate for 30 min on ice Heat shock: 45s at 42 degrees Incubate cells on ice for 2 min Add 1ml LB medium+ 25mM glucose Incubate cells for 1h/1.5h at 37 degrees Spin the cells down, resuspend them in 100μl LB medium and plate 90μl and 10μl out on the plates Put the plates in the stove at 37 degrees overnight Doing plasmid prep of overnight cultures Working on Human practices