IGEM:Groningen/Notebook/iGEM 2011/2011/05/27

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27-5-11 Colonies were seen on the plates with the transformants. Do a colony PCR for samples: RFP, cI 2, GFP RBS, RGV DT, LasR LVA, PLasI, DT 1, GFP Per reaction: Taq 10× buffer: 2μl dNTPs: 0.4μl MgCl2: 1.2μl taq 5u/μl: 0.1μl Forward primer 10mM: 0.4μl Reverseprimer 10mM: 0.4μl MQ: 15.5μl Analyse them on a 1% agarosegel Let colonies grow overnight some time and than isolate plasmids for sequencing.