IGEM:Groningen/2009/Notebook/iGEM 2011/2011/07/06

From OpenWetWare

Jump to: navigation, search
iGEM Project name 1 Main project page
Previous entry      Next entry

Entry title

  • Colony PCR of 6 constructs!

HybB promotor in pSB1CR HybB promotor in RBS GFP ampicillin resistance vector PcI promotor in RBS GFP ampicillin resistance vector PlasI promotor in RBS GFP ampicillin resistance vector LasI-LVA in ampicillin resistance with double terminator vector cI-LVA in ampicillin resistance with double terminator vector

Colony PCR protocol: Media: Taq PCR mastermix Preheated Lid: 111° C Denaturation: 95 ° C for ten minutes Cycle 33×

Denaturation: 95° C for 30 seconds
Annealing: 60° C for 30 seconds
Extension: 72° C for 1 minute

Final extension: 72° C for ten minutes Store at 4 ° C (infinite)



Personal tools