IGEM:Cambridge/2008/Turing Pattern Formation/Constructs

Lowercase letters are biobrick ends, uppercase are the actual pieces.

AgrA

atgaattcgcggccgcttctagagATGGAAATCGCACTGGCTACGGACAATCCATATGAGGTTCTGGAACAGGCTAAAAACATGAACGACATCGGCTGCTATTTCCTGGATATTCAGCTGAGCACCGACATCAACGGTATCAAACTGGGCTCTGAAATCCGTAAACATGATCCGGTTGGTAACATTATTTTCGTGACTTCTCATTCTGAGCTGACCTATCTGACGTTCGTATACAAGGTTGCAGCCATGGATTTCATCTTTAAAGACGATCCGGCAGAGCTGCGTACGCGCATCATCGACTGTCTGGAAACTGCGCACACGCGCCTGCAACTGCTGTCCAAAGATAACTCCGTGGAAACCATTGAACTGAAACGTGGCAGCAACAGCGTTTACGTTCAGTACGATGATATCATGTTCTTCGAAAGCTCCACTAAGTCCCACCGTCTGATCGCGCATCTGGATAATCGTCAGATCGAGTTCTATGGCAACCTGAAAGAGCTGAGCCAGCTGGACGATCGCTTCTTCCGTTGTCATAACTCCTTCGTTGTTAACCGTCACAACATCGAATCCATCGACTCCAAAGAGCGTATTGTATACTTCAAGAACAAAGAACACTGTTATGCGTCTGTACGCAATGTCAAGAAAATCTAAtactagtagcggccgctgcaggcggc

AgrB

atgaattcgcggccgcttctagagATGAACTATTTTGACAACAAGATCGACCAATTTGCTACTTACCTGCAAAAACGCAACAACCTGGACCACATCCAGTTTCTGCAAGTTCGTCTGGGCATGCAGGTTCTGGCTAAAAACATCGGCAAACTGATCGTTATGTACACCATCGCCTATATTCTGAACATCTTCCTGTTTACCCTGATCACCAACCTGACCTTCTATCTGATTCGCCGTCATGCCCACGGCGCTCATGCACCTTCTTCCTTTTGGTGTTATGTAGAGAGCATTATTCTGTTTATCCTGCTGCCGCTGGTAATCGTTAACTTCCACATCAATTTCCTGATTATGATCATCCTGACTGTGATTTCCCTGGGTGTAATCTCTGTTTACGCTCCGGCTGCCACTAAGAAAAAGCCGATTCCGGTGCGCCTGATCAAACGTAAAAAGTACTACGCTATCATCGTTTCCCTGACCCTGTTCATTATCACCCTGATTATCAAAGAACCGTTTGCACAGTTCATCCAGCTGGGTATTATCATCGAAGCGATCACTCTGCTGCCGATCTTTTTCATCAAAGAGGATCTGAAATAAtactagtagcggccgctgcaggcggc

AgrC

atgaattcgcggccgcttctagagATGATTCTGATGTTCACCATTCCAGCAATTATTAGCGGTATTAAGTACAGCAAGCTGGATTATTTCTTCATCATCGTAATCTCTACCCTGTCCCTGTTCCTGTTCAAAATGTTCGATTCCGCGAGCCTGATTATCCTGACTTCCTTCATCATCATCATGTATTTCGTAAAAATCAAATGGTATAGCATCCTGCTGATCATGACCTCCCAGATTATCCTGTACTGTGCCAACTACATGTATATCGTTATCTACGCGTACATCACGAAAATCTCCGATTCTATCTTCGTTATCTTTCCGTCTTTCTTCGTGGTGTACGTTACGATCTCTATTCTGTTTTCCTATATTATCAATCGCGTTCTGAAAAAGATCTCTACTCCGTACCTGATTCTGAACAAAGGTTTTCTGATCGTCATCTCCACTATCCTGCTGCTGACCTTCTCTCTGTTCTTCTTTTACTCTCAGATCAACTCCGATGAGGCAAAAGTCATCCGTCAGTACAGCTTCATCTTTATCGGTATTACTATCTTCCTGAGCATTCTGACTTTCGTTATCAGCCAGTTCCTGCTGAAAGAAATGAAGTACAAGCGCAACCAAGAAGAAATCGAGACTTACTACGAGTACACCCTGAAGATTGAAGCGATCAACAACGAAATGCGTAAATTTCGTCACGACTACGTTAACATTCTGACCACCCTGAGCGAATACATCCGTGAAGACGACATGCCTGGCCTGCGTGATTATTTCAACAAAAACATTGTGCCGATGAAAGATAACCTGCAAATGAACGCTATTAAGCTGAACGGTATCGAAAACCTGAAAGTACGTGAGATCAAGGGTCTGATTACCGCTAAAATCCTGCGTGCGCAGGAAATGAACATCCCAATTAGCATCGAAATCCCAGACGAAGTTTCTAGCATTAACCTGAACATGATTGACCTGTCTCGTAGCATCGGCATCATCCTGGACAACGCTATCGAAGCTTCCACTGAAATCGACGACCCGATTATTCGCGTTGCTTTTATCGAGAGCGAAAACTCCGTGACCTTTATCGTGATGAACAAATGCGCGGACGATATCCCGCGTATTCACGAACTGTTCCAGGAATCTTTCAGCACCAAGGGTGAAGGTCGCGGCCTGGGCCTGTCCACCCTGAAAGAGATCGCGGATAACGCCGACAACGTTCTGCTGGACACTATTATCGAAAACGGTTTCTTCATTCAGAAAGTCGAAATCATCAATAACTAAtactagtagcggccgctgcaggcggc

AgrD

atgaattcgcggccgcttctagagATGAATACTCTGTTCAATCTGTTTTTCGATTTCATTACTGGCATCCTGAAGAACATCGGTAACATCGCAGCGTACAGCACCTGCGACTTCATCATGGATGAAGTAGAGGTCCCGAAAGAGCTGACCCAGCTGCATGAATAAtactagtagcggccgctgcaggcggc

pXyl Promoter

With BB sites: 163 Without: 115

atgaattcgcggccgcttctagagGAAAAACTAAAAAAAATATTGAAAATACTGATGAGGTTATTTAAGATTAAAATAAGTTAGTTTGTTTGGGCAACAAACTAATGTGCAACTTACTTACAATATGACATAAAATGCAtactagtagcggccgctgcagatc

pSpac Promoter

With BB sites: 155 Without: 107

atgaattcgcggccgcttctagagAGAACAACCTCTGCTAAAATTCCTGAAAAATTTTGCAAAAAGTTGTTGACTTTATCTACAAGGTGTGGCATAATGTGTGGAATTGTGAGCGGATAACAATTAAGCTTtactagtagcggccgctgcagata

pPac Promoter

With BB sites: 125 Without: 75

atgaattcgcggccgcttctagagAAACGAGGTCATCATTTCCTTCCGAAAAAACGGTTGCATTTAAATCTTACATATGTAATACTTTCAAAGACTACAtactagtagcggccgctgcaggcggc

pUpp Promoter

With BB sites: 285 Without: 239

gaattcgcggccgcttctagagATTTTTAAAGTATGTATACAAATGATGAATAAATTTTGGCGATATAATGAAGGATACAGCTCCCATAATTGGTAAAGATACTAGATAGATTCATCGTAAAATCATGATTTTGCCAAATTTGCCCTTGAATATTAGTAGCGTTTTCTTTACAATCGTAAATAGTGTAAAAAAGCGTGCAAACGCATGAATATCATCTAAAGGAGAGATTCACATGGGAAAACTGTATGTATTTGATCCTCtactagtagcggccgctgcagat

consensus B. subtilis RBS

With BB sites: 54 Without: 11

gaattcgcggccgcttctagagAAAGGAGG TGTtactagtagcggccgctgcag

weak B. subtilis RBS

With BB sites: 54 Without: 11

gaattcgcggccgcttctagagAGAGGTGG TGTtactagtagcggccgctgcag