IGEM:Cambridge/2008/Notebook/Voltage/2008/08/07

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Flame Photometry Various concentrations of KCl were made up in LB to calibrate for LB instead of water. Took OD600 readings of mutants grown in KCl concentrations in shaking incubator overnight They were then spun down, resuspended in SDW and spun again to wash, then resuspended. The resuspended cells were put through the flame photometer to discern internal cell K+ concentration. The supernatant K+ concentration was also measured. Kdp Ran ligation mixtures on agarose gel and extracted 8kb (supercoiled plasmid) bands. Transformed cells with cleaned up plasmid. Mutants