IGEM:Cambridge/2008/Notebook/Voltage/2008/07/21

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Wet Work Aims for Today The results of yesterday's modified protocol still yielded no growth on the plates. Using yesterday's PCR product our aims were: Cut promoters with spe1 Cut RBS with Xba1 Ligate these together and perform further PCR amplification of linear fragments. Cut first 'stop' with spe1 Cut second 'stop with xba1 Ligate these together and perform further PCR amplification of linear fragments. Finally, put all the above elements into a vector plasmid. End of day: Aims completed Length Promoter = 35 bases RBS = 16 bases Stop (B1002) = 35 bases and Stop (B1006) = 39 bases