IGEM:Cambridge/2008/Notebook/Voltage/2008/07/21

From OpenWetWare

Jump to: navigation, search
........... Electric Output Main project page
Previous entry      Next entry

Wet Work

Aims for Today

The results of yesterday's modified protocol still yielded no growth on the plates. Using yesterday's PCR product our aims were:

  • Cut promoters with spe1
  • Cut RBS with Xba1
  • Ligate these together and perform further PCR amplification of linear fragments.


  • Cut first 'stop' with spe1
  • Cut second 'stop with xba1
  • Ligate these together and perform further PCR amplification of linear fragments.


  • Finally, put all the above elements into a vector plasmid.


  • End of day: Aims completed

Length

  • Promoter = 35 bases
  • RBS = 16 bases
  • Stop (B1002) = 35 bases and Stop (B1006) = 39 bases


Retrieved from "http://openwetware.org/wiki/IGEM:Cambridge/2008/Notebook/Voltage/2008/07/21"
Personal tools