IGEM:Cambridge/2008/Notebook/Magnetic Bacteria/2008/07/28
Magnetic Bacteria | Main project page Previous entry Next entry |
Plasmid AssemblyFur- MutantBrief Introduction of Fur Iron is an important nutrient for E.coli but it presents problems of toxicity at high concentration, poor solubility and low availability. Escherichia coli achieves iron homeostasis through regulation of numerous iron acquisition systems and iron storage proteins. This regulation is mediated by the Fur (ferric uptake regulation) protein, which generally acts as a repressor that utilises ferrous iron as a co-repressor. Fur also regulates genes involved in other functions such as acid shock response, defence against oxygen radicals, and virulence, and is therefore regarded as a global regulator. Thus, fur- mutants which have higher free iron ion concentration in the cytoplasm are to be used for the formation of magnetite in vivo. Preparing fur- Mutants Colonies
Preparing Biobricks<html><b>Gene (mms6 or mamC):</b><br><img src="http://openwetware.org/images/9/98/Genecam.jpeg" align="middle" width="300" > <br><br> </html> Annotated APE file for pSB2K3<html><br><br><br><br><br><br><br><br><br></html> Plasmid pSB3K3: http://partsregistry.org/Part:pSB3K3 Plan:
Restriction Digestion of I0500 and pSB3K3
Result:
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