IGEM:Cambridge/2008/Notebook/Magnetic Bacteria/2008/07/24

From OpenWetWare

Jump to: navigation, search


Magnetic Bacteria Main project page
Previous entry      Next entry

Recovery of mms6 and mamC

Recover mms6 and mamC plasmids from colonies:

  • Recover plasmids from single colonies using Zyppy kit following standard protocol
  • Restriction digest with EcoRI and PstI
  • Run on gel and recover mms6 and mamC DNA


Lane 2 - Hyperladder I
Lane 3 - 22 (Voltage)
Lane 4 - 26 (Voltage)
Lane 5 - mamC
Lane 6 - mms6


Result:

  • Two clear bands obtained on gel at around 400-500 bp
  • Gel cut out and put in eppendorf tubes for gel recovery tomorrow


Gel showing mamC and mms6 as 2 bright bands on lanes 5 and 6
Gel showing mamC and mms6 as 2 bright bands on lanes 5 and 6
Personal tools