IGEM:Cambridge/2008/Notebook/Bacillus/2008/09/01

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Result for Bacillus transformation (27/08) Strain Vector Antibiotic number of colonies Conclusion IA751 None Spc50 0 ok IA751 ECE153 Spc50 about 200 very good efficiency IA751 None Blank confluent cells are alive IA751 None Cm5 a lot problem of contamination (bacillus??? or E.coli???) IA751 ECE112 Cm5 a lot to check New Bacillus transformation (to make glycerol stocks) 2 attempts with plates from 12/08 and 18/08, no growth... Cells may be dead. New plate of IA771 and IA751 (with glycerol stocks)