IGEM:Cambridge/2008/Notebook/Bacillus/2008/08/22

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Stock ECE112, 166, 153

- Plasmid miniprep (2 tubes of ECE112, 2 tubes of ECE166 and 1 tube of ECE153)

- Nanodrop

Transformation of Bacillus

  • For strain IA751 : transform with ECE166, 171, 153 and 112
  • For strain IA771 : transform with ECE166, 171
  • for glycerol stocks (one with 100μL of medium B and glycerol, and one with only glycerol) : transform with ECE166


- We used the same protocol than before with a few changes :

  • add sterile 50% glucose in medium A
  • don't keep samples after using them for spectrophotometer
  • spin each tubes and keep only 100μL of medium A (to concentrate cells) before adding DNA
Time (min) 0 20 40 60 80 100 120 130 140
OD650 for IA751 0.1817 0.1870 0.1929 0.2115 0.2543 0.3291 0.3695 0.4188 0.4224
OD650 for IA751 0.1818 0.1874 0.1907 0.2088 0.2452 0.3182 0.3575 0.4228 0.4314

- For glycerol stocks, resuspend cells into 100μL of medium B, add DNA and wait for 30min, end plate them

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