IGEM:British Columbia/2009/Notebook/Biosensor Sensitivity/2009/07/15

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Glycerol Stock Confirmation

  • PCR Protocol #11
  • Samples: J23100, RBS YFP Terminator, pBad strong RBS RFP, Amp Construction Plasmid, chlor construction plasmid, control
Gel Electrophoresis 1% gel with SYBR green all confirmed!
Material 1 reaction Master mix
10x buffer2.5uL 16.5uL
Vf2 primer1.25uL 8.25uL
Vr primer1.25uL 8.25uL
dNTP 0.5uL 3.3uL
sd water19.3uL 127.38uL
Taq Pol 0.2uL 1.32uL
DNA 0uL 0uL
Total 25uL 165uL


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