IGEM:British Columbia/2009/Notebook/Biosensor Sensitivity/2009/05/21

iGEM Project name 1

Main project page Previous entry      Next entry

Made Antibiotics Kanamycin 50mg/mL stocks; 20 aliquots of 1 mL each, therefore 20 mL Dissolved 1000 mg (1 g) of Kanamycin into 20 mL of water Aliquoted 1 mL ea. into sterile microfuge tubes, freezed at -20ºC Tetracycline 15mg/mL stocks; 20 aliquots of 1mL each, therefore 20mL Dissolved 300mg (0.3g) of Tetracycline into 10mL of 100% Ethanol and 10mL of sdH2O (i.e. dissolved in 50% EtOH) Aliquoted 1mL each into sterile microfuge tubes wrapped in tin foil to protect from light freezed at -20ºC Made LB Plates Done by standard methods Plated Kan at 50ug/mL final plated dilution