IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/09/10

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9/10

Ethanol
Resting Cell Assay started at 8:00

  • O.D. of cells was adjusted to be approx. 1.0 (+-0.2)
  • Cells were resuspended in 30 ml PBS
  • 3 ml of 20% glucose and 1 ml of 90 g/L Pyruvate (equivelant of .09 g per 1 ml, which, when diluted in 30 ml is equal to 3 g/L) was then added.
  • Samples were taken every 2 hours for 8 hours, and then a 24 hour sample was taken.


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