IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/08/08

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Entry title Digest more LacI on amp backbone with S and P. Digest more RBS-TesA with X and P. Clean and concentrate LacI Run RBS-TesA on a 1% agarose gel. Gel extract on Monday. Reagent RBS-TesA (μL) LacI (μL) DNA 15.0 15.0 10X fast Digest Buffer 2.0 2.0 PstI 1.0 1.0 XbaI 1.0 .0 SpeI 0.0 1.0 H2O 1.0 1.0 37°C for 1 hour