Haynes Lab:Notebook/TB Biosensor/2012/10/31

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Entry title Promega GoTaq Green master mix (contains dNTP’s, Taq, MgCl2, and buffer). DNA template 0.4 μl Forward primer (10 μM) 1.0 μl Reverse primer (10 μM) 1.0 μl 2x GoTaq master mix 25 μl dH2O 22.6 μl   50 μl PCR reaction: 95°C/ 3 min. (95°C/ 30 sec; 55°C*/ 30 sec; 72°C/ ___ sec**) x30 cycles 72°C/ 3 min. 4°C/ ∞