Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/06/10
|K562 Transfection Results & RNA Miniprep|| Main project page|
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Day 2 of collecting flow cytometry data, fluorescence microscopy data, and harvesting cells for RNA.
Also need to set up two more samples for a "no plasmid" control transfection. Current cell density of K562 culture is 420,000 cells/mL. Need to wait until culture is around 800,000 cells/mL before proceeding with mock transfection.
Following the protocol for RNA miniprep found here.
Well 4: pMaxGFP, post-48hr
Well 5: KAH126-MV2, post-48hr
Well 6: KAH126-MV2, post-48hr
RNeasy / Trizol
Samples are stored at -80 °C.