Check ligations from 11/20/12 to see if any colonies contained the insert
Used 4 colonies from H2B, 4 colonies from LOV plates
Each colony liquid culture followed the digestion table accordingly
Reagent
H2B
LOV
DNA
5.0
5.0
EcoRI
1.0
0
PstI
0
1.0
10x buffer
1.5
1.5
dH2O
7.5
7.5
Total vol.
15.0
15.0
Run entire 15 μL on 1% gel
Expect 2 bands for H2B and 3 bands for LOV
Results of the enzyme digests are shown, with four H2B bands on the left, and three LOV bands on the right. The gel was loaded with 30 μL samples. The bands are at 2000 bp, confirming the vector self ligated and there is no insert.