Haynes Lab:Notebook/HPK-CFP insertion into Gal4EED/Luc using CRISPR/2015/04/28

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Transformation from DBN004_pSB1A3 Ligation Main project page
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Overview

I'll be checking the plates for colonies and starting liquid cultures & plates from the samples if colonies appear.

Transformation Results

Plate # colonies
neg. control0
colony 110
colony 212
colony 54

Liquid Cultures & Streaks

Four colonies were picked from each transformation plate. They're numbered 1.1, 1.2, 1.3, 1.4, 2.1 etc. where the first number is the original (source) colony and the second number is the colony picked today. There are twelve colonies picked in total, streaked on a library plate and placed in 5mL aliquots of LB + Amp.


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