Haynes Lab:Notebook/Engineering PC-TFs/2014/11/26

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Summary Made Amp Plates CMV/MV9: Cut with XbaI; Gel Purified; Desphos'd BL01/Bl05: Cut with XbaI & SpeI; Gel Purified Reagent Volume Expected: CMV/MV9 (Well 1): 5200 BL01 (Well 3): 2500, 3300 BL05 (Well 5): 2500, 3300 Top: CMV/MV9; Bottom: BL01 (Well 1) & BL05 (Well2); 30 μL/lane; 1% agarose; Ladder Observed lengths: CMV/MV9: 5200 BL01: 2500 (desired) BL05: 2500 (desired) DNA(CMV/MV9, BL01, BL05) 15.0 μL 10X buffer 3.0 μL XbaI 2.0 μL SpeI (no SpeI for CMV/MV9) 2.0 μL dH2O 8 (10 for CMV/MV9) μL Total 30 μL --> 37°C/ 30 min. Concentration Readings Gel purified BL05 400px|Hover name