Haynes Lab:Notebook/Engineering PC-TFs/2014/11/26

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Summary

  • Made Amp Plates
  • CMV/MV9: Cut with XbaI; Gel Purified; Desphos'd
  • BL01/Bl05: Cut with XbaI & SpeI; Gel Purified


Reagent Volume

Expected:
CMV/MV9 (Well 1): 5200
BL01 (Well 3): 2500, 3300
BL05 (Well 5): 2500, 3300


Today's gelToday's gel

Top: CMV/MV9; Bottom: BL01 (Well 1) & BL05 (Well2);
30 μL/lane; 1% agarose; Ladder

Observed lengths:
CMV/MV9: 5200
BL01: 2500 (desired)
BL05: 2500 (desired)

DNA(CMV/MV9, BL01, BL05) 15.0 μL
10X buffer 3.0 μL
XbaI 2.0 μL
SpeI (no SpeI for CMV/MV9) 2.0 μL
dH2O 8 (10 for CMV/MV9) μL
Total 30 μL --> 37°C/ 30 min.


Concentration Readings

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Gel purified BL05

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