Haynes Lab:Notebook/Engineering PC-TFs/2014/10/13

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Summary

  • RFP
  • Bradford

Plate reader measurements

RFP signal

  1. U2OS BL09 = 181
  2. Mock Transfection = 25


Bradford assay

  1. Standard 1 (0 μg) = 0.276
  2. Standard 2 (1 μg) = 0.234
  3. Standard 3 (2 μg) = 0.394
  4. Standard 4 (4 μg) = 0.443
  5. Standard 5 (8 μg) = 0.644
  6. Standard 6 (16 μg) = 0.829
  7. U2OS BL09 = 0.313
  8. Mock Transfection = 0.302


Standard curve


Final results:

SampleProtein μg/μLProtein*100 μLRaw Fluor. (100 uL)Fluor/ μg protein
U2OS BL090.76576.501812.365929
Mock Transfection0.46446.45250.538235



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