Haynes Lab:Notebook/Engineering PC-TFs/2014/08/05

Main project page Previous entry      Next entry

Summary Picked Colonies for Liquid Cultures CMV/MV9, BL01, BL05, BL09, RhlI plates had growth while the negative control had no growth. ALL PcTF COLONIES WERE PICKED From left to right, top to bottom: Experimental plate BL01: One colony. Experimental plate BL05: Four colonies. Experimental Plate Bl09: Six colonies. CMV/MV9 Backbone: Ignore. Did not use the proper plasmid for benchmarking. Positive control RhlI: Lots of growth. Cells seem good. Negative control: No growth. Plates seem good. Grew liquid cultures of the 11 PcTF colonies using 4mL amp resistant LB liquid media. Put in incubator at 37°C and 238RPM for overnight growth.