Summary
Gel Verification
Digest All the Things
Well One: Ladder
Well Two & Three
Reagent
|
Volume
|
Expected: 2559bp, 3226bp Observed: ~3500bp, ~2800bp
|
DNA(BL01 C1, C2) |
2.0 μL
|
10X buffer |
1.5 μL
|
PstI |
1.0 μL
|
EcorI |
1.0 μL
|
dH2O |
9.5 μL
|
Total |
15 μL --> 37°C/ 30 min.
|
Well Five & Six
Reagent
|
Volume
|
Expected: 2559bp, 3226bp Observed: ~3500bp, ~2800bp
|
DNA(BL05 C1, C2) |
2.0 μL
|
10X buffer |
1.5 μL
|
PstI |
1.0 μL
|
EcorI |
1.0 μL
|
dH2O |
9.5 μL
|
Total |
15 μL --> 37°C/ 30 min.
|
Well Four & Seven
Reagent
|
Volume
|
Expected: 1482bp, 3226bp Observed: ~3500bp, ~1500bp
|
DNA(BL09 C1, C2) |
2.0 μL
|
10X buffer |
1.5 μL
|
PstI |
1.0 μL
|
EcorI |
1.0 μL
|
dH2O |
9.5 μL
|
Total |
15 μL --> 37°C/ 30 min.
|
Well Eight & Nine
Reagent
|
Volume
|
Expected: 4552bp, 653bp Observed: ~5000bp, ~700bp
|
DNA(CMV/MV9 C1, C2) |
2.0 μL
|
10X buffer |
1.5 μL
|
PstI |
1.0 μL
|
NruI |
1.0 μL
|
dH2O |
9.5 μL
|
Total |
15 μL --> 37°C/ 30 min.
|
Expected Bands .
|
Observed results.
Ladder
|
|