Haynes Lab:Notebook/Engineering PC-TFs/2014/04/08

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Summary PCR Reaction Reagent Volume Expected: 1. PCR Reaction Product = 845bp DNA(CMV/MV9) 0.5 μL 2X GoTaq 12.5 μL FP DD122 B 1.0 μL RP DD123 B 1.0 μL dH2O 10.0 μL Placed reaction mixture into PCR machine on setting GOTAQ35cyc Gel verification 15 μL/lane; 1% agarose; band are at ~ 845bp (both lanes are the same product). Ladder