Haynes Lab:Notebook/Engineering PC-TFs/2014/04/08

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Summary

  • PCR Reaction
Reagent Volume Expected:
1. PCR Reaction Product = 845bp
DNA(CMV/MV9) 0.5 μL
2X GoTaq 12.5 μL
FP DD122 B 1.0 μL
RP DD123 B 1.0 μL
dH2O 10.0 μL
  • Placed reaction mixture into PCR machine on setting GOTAQ35cyc
  • Gel verification
Hover name
15 μL/lane; 1% agarose; band are at ~ 845bp (both lanes are the same product).
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