Haynes Lab:Notebook/David Barclay Undergrad Training/Plasmid Transformation/2015/09/01

Haynes BioBrick Cloning

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Growing Liquid Cultures Negative control from 8-31-15 grew 3 colonies so colonies for BD004 could not be trusted Picked colonies from streak plate from 8-3-15 for both BD004 MV2 and BD006 MV2 Liquid Cultures Culture1-4=colony 1 from plate 1 BD004/MV2 Culture 2-4= colony 2 from plate 1 BD004/MV2 Streak plate B0004 colonies 1 and 2 Culture 1-6 = colony 1 from plate 1 BD006/MV2 Culture 2-6 = colony 2 from plate 1 BD006/MV2 Streak plate B0006 colonies 1 and 2