Haynes Lab:Notebook/David Barclay Undergrad Training/Plasmid Transformation/2013/10/01

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Day 3- Minipreps fshPCD

Liquid Culture Results

NOTE: Cultures and Streaks taken out after 18 hours (37 incubator) and left on lab bench for 1 hour.

fshPCD

  • Cultures Innoculated 09/27/13
  • Cultures 1 and 2: Both show growth
  • Streak 1 and 2: Both show growth

Samples for Minipreps and Analysis

  • Sample 1= fshPCD/V0120 colony 1; BBa_J176002; part= 186 bp; vector= 4968 bp
  • Sample 2= fshPCD/V0120 colony 2; same

DNA Concentration Data

Plasmid OD260 OD260/280 ng/μL
1. fshPCD 1 0.014 1.714 24.682
2. fshPCD 2 0.013 1.762 28.523

Did not have good concentration, nothing else done after this point



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