Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/02/26

From OpenWetWare

Jump to: navigation, search
Project name Main project page
Previous entry      Next entry


Since the sequencing results failed, we decided to troubleshoot and find out whether our error is with the primers or the plasmid.
I performed a digestion again with XbaI and PstI. I created a reaction for each plasmid sample that I mini prepped on Monday.
Reactions are as follows:

Thing Vol
0.25ug DNA sample7.5 uL
XbaI0.5 uL
PstI0.5 uL
Green digest buffer2 uL
Water9.5 uL

After pipetting contents into each tube, I incubated the reactions for 15 minutes at 37°C.
The next step is to run a gel with each reaction and see if we get the desired results.

Personal tools