Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/05/19

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10:1 control plate has 0 colonies, 10:1 LuxR receiver plate has maybe 7 or so colonies (some are very small, and several of the plates have many colony-sized bubbles making it hard to count). Picked four colonies off X 10:1 plate to miniprep tomorrow.

Golden gate ligations for other receivers with 10:1 insert to bb ratio

Reaction Rec Bb pLux-GFP pLac-LuxR BsmBI T4 Ligase T4 Ligase buffer H2O
Bb ctrl0.74000.51.2525.51
Rhl Rec0.742.781.680.51.2521.05
Esa Rec0.744.352.70.51.252-1.54

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