Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/04/21

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Testing the GG constructs miniprepped on 4/11/14 to see if there was something weird with the sequencing primer binding site, like maybe it was relocated or duplicated during cloning (one of the GG primer binding sites overlaps the sequencing primer binding site). Template numbers correspond to the numbers Behzad added to the tubes. Ran 20ul reactions, 1-4 on Wang lab PCR machine, 5-12 on Haynes lab machine. 0.5 ul of each template.

Sample # Primer 1 Primer 2 Template Annealing T
1P103P104549
2P103P104649
3P103P104749
4P103P104949
5P105P107561
6P105P107661
7P105P107761
8P105P107961
9P111P112561
10P111P112661
11P111P112761
12P111P112961


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