01/08/2015
Ran the PCR reactions from yesterday on a gel. They look great, no extra bands.
Purified the PCR products using Sigma's PCR clean up kit. Eluted in 50ul elution solution.
Sample Read#
|
260
|
280
|
260/280
|
ng/µL
|
Untreated |
0.107 |
0.057 |
1.862 |
106.778
|
23 |
0.096 |
0.051 |
1.889 |
96.073
|
25 |
0.1 |
0.054 |
1.846 |
100.394
|
29 |
0.104 |
0.055 |
1.889 |
104.281
|
30 |
0.099 |
0.053 |
1.862 |
99.101
|
32 |
0.093 |
0.05 |
1.867 |
93.338
|
33 |
0.091 |
0.048 |
1.875 |
90.77
|
34 |
0.093 |
0.051 |
1.845 |
93.316
|
36 |
0.094 |
0.051 |
1.854 |
93.841
|
39 |
0.097 |
0.053 |
1.847 |
97.225
|
43 |
0.089 |
0.047 |
1.901 |
88.782
|
44 |
0.03 |
0.016 |
1.889 |
30.391
|
45 |
0.087 |
0.047 |
1.861 |
86.634
|
46 |
0.079 |
0.044 |
1.814 |
79.106
|
Set up annealing reaction for the PCR samples
Sample
|
ul for 400ng
|
ul H2O
|
ul anneal buff
|
Untreated |
3.75 |
9.75 |
1.5
|
23 |
4.16 |
9.34 |
1.5
|
25 |
3.98 |
9.52 |
1.5
|
29 |
3.84 |
9.66 |
1.5
|
30 |
4.04 |
9.46 |
1.5
|
32 |
4.29 |
9.21 |
1.5
|
33 |
4.41 |
9.09 |
1.5
|
34 |
4.29 |
9.21 |
1.5
|
36 |
4.26 |
9.24 |
1.5
|
39 |
4.11 |
9.39 |
1.5
|
43 |
4.51 |
8.99 |
1.5
|
44 |
13.16 |
0.34 |
1.5
|
45 |
4.62 |
8.88 |
1.5
|
46 |
5.06 |
8.44 |
1.5
|
Put at -20 after they finished
Set up PCR wiht phusion for the remaining gRNA treated samples.
|