Group 14

BME 103 Fall 2012	Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Anshul Krishnan Role(s) Name: Sara Bacon Role(s) Name: Lydia Gabric Role(s) Name: Charmayne Seaton Role(s) Name: Christopher-Noel Ward Role(s) LAB 1 WRITE-UP The goal of our group is to develop a solution for an easy, at-home diagnostic test for Chlamydia and Gonorrhea. In 2015, the CDC reports that in every 100,000 people, 479 reported having Chlamydia. The same study showed that in the same number of people, 124 reported being affected by Gonorrhea (Centers for Disease Control and Prevention, 2016). The main idea behind this concept is to create a test, much like a pregnancy test, that can be completed at home and only requires a urine sample. Chlamydia and Gonorrhea are often tested together at facilities due to the fact that they are considered co-existing infections that are both caused by a bacteria. Both are sexually transmitted diseases. Typically, the infection remains in the urogenital tract, and therefore can be tested using a urine sample (The Editors of Encyclopædia Britannica, 2011). Customer Validation 1) AHRQ or U.S. Department of Health and Human Services 2) The U.S. Preventive Services Task Force (USPSTF) 3) Centers for Disease Control and Prevention 4) U.S. Preventive Services Task Force 5) American Congress of Obstetricians and Gynecologists 6) Planned Parenthood 7)National Institute of Allergy and Infectious diseases 8)American Sexual Health Association 9)Kinsey Institute, Indiana University 10) Medical Institute for Sexual Health 11)Yale Medicine 12) Dr. Pankaj Chopra, MD 13) Dr. Ehreema J. Nadir, MD Chandler Regional Medical Center 14) Alpha Health STD Screening 15) mLab Box 16) Walgreens 17) Walmart 18) CVS 19) Target 20) Albertsons Competitors Current Diagnostics 1) At home testings sent to Labs - Advantage: Privacy - Disadvantage: Costs, wait time for results 2) At Lab testings -Advantage: On the Spot Results -Disadvantage: Cost Our device is more marketable due to a number of reasons. First, it gives a sense of privacy to all consumers. It will be available at all drugstores and pharmacies, making it easily accessible. It also reduces both the cost and wait time for results. Anyone can buy one to get tested. It combines both at home and at lab testings into one ideal examination. IP Position US5516638A: Immunoassays for the detection of antibodies to Chlamydia trachomatisi in the urine. Legal Status: Expired (Lifetime) Inventors: Howard B. Urnovitz, Toby D. Gottfried, David J. Robison Current Assignee: Calypte Inc. Grant Date: 1996-05-14 US44463090A: Cascade amplification enzyme immunoassay Legal Status: Expired (Fee Related) Inventor: Curtis. C Harris Current Assignee: Rapidex Ltd. Grant Date: 1984-7-31 Abstract: The invention provides enzyme immunoassays whose sensitivity is increased by cascade amplification. The coupled ligand (enzyme or an activator) catalytically activates a second enzyme which acts on a substrate or can act on a third enzyme to produce a cascade. Alternatively, a proenzyme is coupled to the ligand and converted by an activator to an enzyme which is itself an activator of a second proenzyme in a cascade. US4299916A: Preferential signal production on a surface in immunoassays Legal Status: Expired (Lifetime) Inventors: David J. Litman ,Edwin F. Ullman Current Assignee: Siemens Healthcare and Diagnostics Grant Date: 1981-11-10 Abstract: An assay method and compositions are provided for determining the presence of an analyte in a sample. The analyte is a member of an immunological pair (mip) of immunogens--ligand and receptor. The method has two basic elements: a solid surface to which one of the members of the immunological pair is bonded and a signal producing system, which includes a catalytic member bonded to a mip, which signal producing system results in a measurable signal on said solid surface related to the amount of analyte in the medium. The signal generating compound is produced without separation of the catalyst labeled mip bound to the solid surface from the catalyst labeled mip free in solution. In a preferred embodiment, an en zyme is bonded to a mip which acts in conjunction with a solute to produce a signal generating product which binds preferentially to the solid surface when the enzyme is bound to the surface, resulting in a signal which is readily differentiated from signal generating compound produced by the catalyst and solute in the bulk solution. Fundability Competitors 3 - Once released, such a STD test would be sought after by all sorts of departmental stores and pharmacies; Those who are sexually active would now be willing to get tested as there is no issues with privacy and complications with the process. Easily replacing all of the "At Lab" alternatives for STD testing Customer Validation 3- Once released, such a STD test would be sought after by all sorts of departmental stores and pharmacies; IP Position 2- There have been patents issued for the technology that our device uses, but they've either expired or have been inactive for a large amount of time and can be bought over with a small fee Initial Machine Testing The Original Design (Add image of the full OpenPCR machine here, from the Week 3 exercise. Write a paragraph description for visitors who have no idea what this is) Experimenting With the Connections When we unplugged (part 3) from (part 6), the machine ... (did what? fill in your answer) When we unplugged the white wire that connects (part 6) to (part 2), the machine ... (did what? fill in your answer) Test Run (Write the date you first tested Open PCR and your experience(s) with the machine) Protocols Polymerase Chain Reaction (Add your work from Week 3, Part 1 here) Flourimeter Measurements (Add your work from Week 3, Part 2 here) Research and Development Specific Cancer Marker Detection - The Underlying Technology (Add a write-up of the information discussed in Week 3's class) (BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.) Results (Your group will add the results of your Fluorimeter measurements from Week 4 here)