GFP

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Contents

General Info

  • From jellyfish Aeqorea victoria
  • wtGFP sequence on NCBI
  • 238 amino acids
  • The fluorophore is tyrosine Y66 and the surrounding amino acids of Ser(65)-Tyr(66)-Gly(67) are also critical [1].
  • Oxygen is required for maturation of the fluorophore

GFP Variants

  • GFPmut3b - S65G, S72A
  • GFPmut3* - mutations from wt: S2R,S65G,S72A
    • This is GFPmut3b[2] with an accidental mutation at position 2 that doesn't affect function according to the authors.[3]
  • Emerald - F64L, S65T, S72A, N149K, M153T, I167T
  • EGFP - added GTG as second codon, F64L, S65T + optimized for human codons with 35-fold increase in fluorescence over GFP [4]
  • Other variants and their mutations can be found in Shaner supplementary table 2 [5]

Mutations

  • F64L: improve folding @37C
  • S65T: 5-6x increase in amplitude and red shift
  • S65(G|T) and T203(Y|F|W|H) -> YFP
  • Y66W: GFP -> CFP
  • Y66H: GFP -> BFP (more blue than CFP), dim, easily photobleached
  • Y66F: excitation 360nm, emission 442nm
  • R96A: slows cyclization reaction from minutes to months
  • Y203I: eliminate excitation peak at 475nm, leaving lower peak of 399nm. emission remains at 511nm, producing large Stokes shift
  • A206K: make monomeric

References

General

  1. Molecular Biology and Muation of Green Fluorescent Protein (Book Chapter, Zacharias & Tsien) [zacharias]
  2. Cormack BP, Valdivia RH, and Falkow S. FACS-optimized mutants of the green fluorescent protein (GFP). Gene 1996; 173(1 Spec No) 33-8. pmid:8707053. PubMed HubMed PubGet [Cormack]
  3. Andersen JB, Sternberg C, Poulsen LK, Bjorn SP, Givskov M, and Molin S. New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Appl Environ Microbiol 1998 Jun; 64(6) 2240-6. pmid:9603842. PubMed HubMed PubGet [Andersen]
  4. Li X, Zhang G, Ngo N, Zhao X, Kain SR, and Huang CC. Deletions of the Aequorea victoria green fluorescent protein define the minimal domain required for fluorescence. J Biol Chem 1997 Nov 7; 272(45) 28545-9. pmid:9353317. PubMed HubMed PubGet [li97]
  5. Shaner NC, Steinbach PA, and Tsien RY. A guide to choosing fluorescent proteins. Nat Methods 2005 Dec; 2(12) 905-9. doi:10.1038/nmeth819 pmid:16299475. PubMed HubMed PubGet [shaner05]
  6. Prasher DC, Eckenrode VK, Ward WW, Prendergast FG, and Cormier MJ. Primary structure of the Aequorea victoria green-fluorescent protein. Gene 1992 Feb 15; 111(2) 229-33. pmid:1347277. PubMed HubMed PubGet [prasher]
    original cloning of GFP

  7. Chalfie M, Tu Y, Euskirchen G, Ward WW, and Prasher DC. Green fluorescent protein as a marker for gene expression. Science 1994 Feb 11; 263(5148) 802-5. pmid:8303295. PubMed HubMed PubGet [chalfie]
    original use of GFP as a reporter

  8. Tsien RY. The green fluorescent protein. Annu Rev Biochem 1998; 67 509-44. doi:10.1146/annurev.biochem.67.1.509 pmid:9759496. PubMed HubMed PubGet [tsien98]
    good review of GFP

All Medline abstracts: PubMed HubMed

GFP as a measure of gene expression

  1. Leveau JH and Lindow SE. Predictive and interpretive simulation of green fluorescent protein expression in reporter bacteria. J Bacteriol 2001 Dec; 183(23) 6752-62. doi:10.1128/JB.183.23.6752-6762.2001 pmid:11698362. PubMed HubMed PubGet [Lindow]
  2. Iafolla MA, Mazumder M, Sardana V, Velauthapillai T, Pannu K, and McMillen DR. Dark proteins: effect of inclusion body formation on quantification of protein expression. Proteins 2008 Sep; 72(4) 1233-42. doi:10.1002/prot.22024 pmid:18350571. PubMed HubMed PubGet [Iafolla]
  3. Bagh S, Mazumder M, Velauthapillai T, Sardana V, Dong GQ, Movva AB, Lim LH, and McMillen DR. Plasmid-borne prokaryotic gene expression: sources of variability and quantitative system characterization. Phys Rev E Stat Nonlin Soft Matter Phys 2008 Feb; 77(2 Pt 1) 021919. pmid:18352063. PubMed HubMed PubGet [Bagh]
  4. Dong GQ and McMillen DR. Effects of protein maturation on the noise in gene expression. Phys Rev E Stat Nonlin Soft Matter Phys 2008 Feb; 77(2 Pt 1) 021908. pmid:18352052. PubMed HubMed PubGet [Dong]
All Medline abstracts: PubMed HubMed
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