SC medium contains yeast nitrogen base, ammonium sulfate, a carbon source (usually glucose) and amino acids. Depending on the specific strains and experiments different variations are necessary.
Recipe for 1 liter
- 6.8 g Yeast nitrogen base (YNB, including ammonium sulfate, from Formedium)
- 0.79 g Complete supplement mixture (CSM, from Formedium)
- Fill up to 950 ml and autoclave
- Add 50 ml of a 40% glucose solution that was autoclaved separately
- If solid medium is required, add 20 g/L agar prior to autoclaving
- For SC-URA use 0.77 g/L CSM–URA drop-out mix Qbiogene
- For SC-P use 0.7 g/L phosphate free YNB (Qbiogene). The desired amount of phosphate can then be added separately. We use YNB without ammonium sulfate and potassium phosphate. Therefore, ammonium sulfate must be added separately!
- To test nitrogen sources Yeast Carbon Base (YCB) can be used (from Formedium). YCB already contains 10.15 g glucose! Therefore, add only 10 g glucose for 1 L of medium.