Freimoser:Recipes:DiluentA
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Use
Diluent A is used to dilute NEB T4 ligase [1] to a final concentration of 1U/μl (400x dilution).
Composition
- 10 mM Tris-HCl
- 1 mM Dithiothreitol (DTT)
- 0.1 mM EDTA
- 50% Glycerol
- 200 mg/ml BSA
- 50 mM KCl
Adjust pH to 7.4 (25°C)