File:Detection-of Brucella spp. by-PCR-from-milk-samples isolated from the state of Guanajuato

In the present study, milk samples collected from Cuerámaro, Guanajuato were analyzed by PCR assays in order to show the presence of the pathogenic Bacteria Brucella spp . Assays with attenuated vaccines containing Brucella spp., showed amplicons of ~ 200 bp amplified, corresponding to the expected size of the omp-2 gene of Brucella spp. Nonthless, assays with milk samples showed no amplicons, indicating that there was no presence of Brucella spp., making the milk suitable for consumption. This report shows the potential of PCR assays for the detection of the pathogenic bacteria Brucella in milk and also shows that the JPR and JPF primers are effective in the amplification of the omp-2 gene of Brucella spp.