Protocols
Making Gel for gel electrophoresis
- Make 100ml of gel at at time (e.g. 0.8%, 1%, 1.5%, or 2%)
- Measure out the relevant percentage in agarose (e.g. 2g of agarose for 100ml of 2% gel).
- Fill it up with 1xTAE buffer.
- Microwave on low, constantly taking it out every so often to swirl and mix it. Keep microwaving until clear. Make sure it is completely clear
- Cool to a temperature that is still hot but still can be held in your hand. If the gel gets too cold and starts to harden, start microwaving again until clear.
- Add 3uL of ethidium bromide for every 100ml of gel after cooled to the correct tempearature. Mix it well by swirling
- Pour it onto the molds quickly.
- Wait at least 1/2hr until using it.
Gel electrophoresis
- Select a relevant % agarose gel based on your own experience (general guide: all <1000: 2%, all >2000: 1% guide).
- Load samples in wells.
- Load 7uL of marker into a well.
- Run in the 13x13cm box at 90V or 100V and 400mA for the desired amount of time.
- View in the gel viewer machine.
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iGEM Protocols
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