Etchevers:Notebook/Genomics of hNCC/2008/12/10
Genomics of human neural crest cells | Main project page Previous entry Next entry |
R.I.P. and NBFinally threw away the last remnants of the cells that had been in Rich and Neural medium. There were still cells left in the "neural" culture but they were pretty much pericyte-like and those can hang on for months. The medium had evaporated, meanwhile (after 10 days!). When I was last in Paris I had established 2x C13 new hNCC cultures that Sophie says are growing well.
Looking at neuroblastoma data. In particular, cf. the paper by Chen et al. published at the same time as Janoueix-Lerosey et al. on the CNVs that were identified in NB cell lines.
When I've made the table, will put up on GoogleDocs.
"Thirteen tumors (all from hemizygous mice) showed gains and losses of chromosomal regions (Figure 8, Table II). In mice hemizygous for the MYCN transgene, chromosomal regions were most commonly gained on chromosomes 11 (5/16) and 17 (6/16). These regions are syntenic with human chromosomes 6 and 17 respectively (Copeland et al., 1993), both of which commonly are gained in human neuroblastoma (Plantaz et al., 1997). Chromosomal loss was most often detected on chromosomes 5 (4/16), 9 (3/16), 16 (4/16) and X (4/16). These chromosomes are syntenic with human chromosomes 4, 11, 3 and X respectively (Copeland et al., 1993), all of which commonly are lost in human neuroblastoma (Plantaz et al., 1997)."
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