Etchevers:Notebook/Genomics of hNCC/2008/10/22

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Petit bout de papier

Looked at rabbit cornea for the male 980; it may have been the control rabbit; the R and L corneas looked identical. Some auto-fluorescence in collagen layers (PAF fixed, no anti-aldehyde treatment), scar not obvious to find in either case either. May need to cut more eyes, may need to deparaffinate - but I don't think so.

Took photos of different cultures after fixation on light microscope.

Image:401_20x1.JPG Panserin 401 medium. Cephalic hNCC at 20x.

Image:401_20x2.JPG Panserin 401 medium. Cephalic hNCC at 20x.

Image:C6000_20x.JPG CHO C6000 medium. Cephalic hNCC at 20x.

Image:H-ceph-20x.JPG hEScGro medium. Cephalic hNCC at 20x.

Image:H-T_20x1.JPG hEScGro medium. Truncal hNCC at 20x.

Image:H-T_20x2a.JPG hEScGro medium. Truncal hNCC at 20x, another shot.

Image:H-T_20x2b.JPG hEScGro medium. Truncal hNCC at 20x, another shot.

Image:S-ceph0.JPG Stempan medium. Cephalic hNCC at 20x.

Image:S-ceph1_20xarrows.jpg Stempan medium again, Cephalic hNCC at 20x, arrows showing spaces cells seem to avoid on plastic.

Image:S-ceph2-20xarrow.jpg Stempan medium again, Cephalic hNCC at 20x, arrow showing an apparent neurite or other ramification.

Image:S-ceph4-20x_futureN.JPG Stempan medium then 3 days of neural differentiation medium from Sigma. Cephalic hNCC at 20x.

Image:S-ceph5-20x_futureN.JPG Stempan medium then 3 days of neural differentiation medium from Sigma. Cephalic hNCC at 20x.

  • Heather 05:55, 7 November 2008 (EST):


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