Erickson lab:Notebook/defensin expression/2008/09/15

From OpenWetWare

Jump to: navigation, search
Defensin Expression Main project page
Previous entry      

PCR of CAT/RAT Flea

  • I ran PCR using Cat and Rat flea cDNA made previously and using FNde1 and defensin RBamH1 primers.


10μL Master Mix

2μL each primer(5uM)

1μL cDNA template

5μL water


Ran a gel of the PCR products

Image:Cat_flea_PCR_9.15.08small.TIF

     Lane 1: 2-Log Ladder
     Lane 2 cat flea-infected
     Lane 3 cat flea-uninfected
     Lane 4 rat flea control
     Lane 5 negative control



Personal tools