Endy:F2620/Transfer Curve

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Description

The most important characteristic about a device is its transfer curve. The transfer curve of a device specifies how its output varies with its input.

Protocols & Data

Issues

  • What is the time lag between adding AHL to the wells and bringing the plate to the plate reader? I would guess 10min, but I can do a pipeting exercise and let you know more precisely
  • Why does this surface show less time-varying behavior than the specificity 3-d surfaces we have looked at before. Possibly because each of the single curves for each colony looks much smoother, they are posted online, you can have a look
  • Can we look at the variability in the flow data and use that to predict the difference in means that we would expect for the variability cultures?
    • I think it is good idea but I also think that that would be easiest done using flowjo and just looking at the /sigma or FWHM for the distributions. Could you show me how to do that?
      • Matlab might be the best. I can send you mfiles that will let you import the flow data into matlab.

Sure, could you do that?

  • Are you sure that your highest concentration of AHL enables to saturate the pLux promoter (according to your data)? Have you tried higher concentrations ? Vincent 11:31, 25 November 2006 (EST)
    • BC 11:50, 14 December 2006 (EST): Yes, we've gone one order of magnitude higher in concentration (to 1E-4M AHL) and not seen an increase in output. Sometimes we even see a decrease in output at that high an input level.
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