E. coli genotypes/Exhibit
From OpenWetWare
This is a demo of Exhibit. It only includes some of the data from E. coli genotypes.
<exhibit>
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<facet facetClass="TextSearch" facetLabel="Search"/>
<facet expression=".genotype" />
<facet expression=".purpose" />
<facet expression=".resistance" />
<facet expression=".requires" />
<view viewClass="Tabular"
label="Table"
columns=".label, .genotype, .parent, .purpose"
sortColumn="0"
/>
</exhibit>
| Strain | Description | Genotype | Parent | Requires | Resistance | Purpose | Inducible | Lambda |
|---|---|---|---|---|---|---|---|---|
| AG1 | endA1;recA1;gyrA96;thi-1;relA1;glnV44;hsdR17(rK- mK+) | thiamine | nalidixic acid | cloning | ||||
| BL21(DE3) | F-;ompT;gal;dcm;lon;hsdSB(rB- mB-);lacIq;lambda;lacUV5-T7 gene 1;ind1;sam7;nin5 | B834 | protein expression | T7 RNA polymerase | prophage | |||
| BL21(DE3) pLysS | The pLysS plasmid (with chloramphenicol resistance) encodes T7 phage lysozyme, an inhibitor for T7 polymerase which reduces and almost eliminates expression from transformed T7 promoter containing plasmids when not induced. | F-;ompT;gal;dcm;lon;hsdSB(rB- mB-);lacIq;lambda;lacUV5-T7 gene 1;ind1;sam7;nin5;pLysS(cmR) | BL21(DE3) | chloramphenicol | protein expression | T7 RNA polymerase | prophage |
