Dropbase: Picoinjector

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Overview

Description

• Device for picoinjection of 100 pL droplets
• The height of the channels was 50 µm
• Zig-zag post generation for mixing of droplets
• Droplet injection area contains pillars of decreasing size to filter out fibres or particles from the incoming flow of droplets prior to picoinjection

Reference

Ladeveze et al, Versatile Product Detection via Coupled Assays for Ultrahigh-Throughput Screening of Carbohydrate-Active Enzymes in Microfluidic Droplets, ACS Catal, 2023, doi:0.1021/acscatal.3c01609

Downloads

Picoinjector with filters for 100 pL droplets: png, dwg

Usage Notes

• The last line of pillars is perfectly adapted for 100 pL droplets. We recommend using a spacing oil flow of 5 µL/min for a 3 µL/min droplet flow. The flow of picoinjection solution can be adjusted, but using a similar 3 µL/min flowrate as for the droplets reinjection, we could picoinject of 100 pL to the droplets @ 500Hz. The smallest droplets we could picoinject on this device were 50pL. Using flow rates of 5 µl/min + 1.5 µl/min + 3.5 µl/min for oil, droplets and picoinjected solution respectivelly, we could picoinject a volume of 150pL to 50pL droplets @ 340 Hz. SL