DpnI

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Contents 1 Purpose 2 Procurement 3 Use 3.1 Relevant protocols 4 Safety 5 References 6 Contact

[edit] Purpose

• To digest (Adeno) methylated GATC sites. Useful for removing cell-derived plasmid template from PCR samples.

[edit] Procurement

• Can be ordered from NEB.
• Typically stored at -20°C.

[edit] Use

• Use in NEBuffer 4. However, can be added directly to PCR sample.
• Incubate at 37°C.
• Heat inactivate by incubating at 80°C for 20 minutes.
• DpnI cleaves hemi-adenomethylated dam sites 60X more slowly than fully methylated. NEB FAQ
• Plasmids derived from Dam+ e.coli strains will be a substrate

[edit] Relevant protocols

• Site-directed mutagenesis

[edit] Safety

• None known.

[edit] References

• DpnI from NEB

[edit] Contact

• Reshma Shetty