Diary

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April

17th

the first meeting

Team Todai nanORFEVRE was fixed as the name of our team. Yearly schedule was checked.

We read in turn following two reviews of the structural DNA nanotechnology.

  • Andre V. Pinheiro, Dongran Han, William M. Shih, and Hao Yan Challenges and opportunities for structural DNA nanotechnology
  • Nadrian C. Seeman Nanomaterials Based on DNA

24th

the second meeting

We read in turn following two papers.

  • Paul W. K. Rothemund Folding DNA to create nanoscale shapes and patterns

The summary is as follows: A simple method for folding long, single-stranded DNA into arbitrary two-dimensional shapes was invented. The design for a desired shape is made by rater-filling the shape with scaffold and choosing staple strands to hold the scaffold in place. Once synthesized and mixed, the staple and scaffold strands self-assemble, and desired shapes such as squares and stars can be made.

May

8th

the third meeting

We read in turn following four papers.

  • Qiao Jiang, Chen Song, Jeanette Nangreave, Xiaowei Liu, Lin Lin, Dengli Qiu, Zhen-Gang Wang, Guozhang Zou, Xingjie Liang, Hao Yan, and Baoquan Ding DNA Origami as a Carrier for Circumvention of Drug Resistance

The summary is as follows: DNA origami as carrierThe DNA origami nanostructure, which is biocompatible and have spatially addressable surfaces for multi-functional activity, is presented as a promising drug carrier system in the treatment of cancer. It effectively loaded Doxorubicin, a well known anticancer drug, with itself and exhibited prominent citotoxity not only to regular human breast adenocarcinoma cancer cells (MCF 7), but more importantly to doxorubicin-resistant cancer cells. It seems that the inhibition of the lysosomal acidification, resulting in cellular redistribution of the drug to action sites contributes to that.

  • Martin Langecker, Vera Arnaut, Thomas G. Martin, Jonathan List, Stephan Renner, Michael Mayer, Hendrik Dietz, Friedrich C. Simmel Synthetic Lipid Membrane Channels Formed by Designed DNA Nanostructures

The summary is as follows: We created transmembrane channels in lipid bilayers by DNA origami. Single-channel electrophysiological experiments show that the synthetic channels have similarities to the natural ion channels such as conductances and channel gating. And single-molecule translocation experiments show that the synthetic channels can be used to discriminate single DNA molecules.

  • Masayuki Endo, Koichi Tatsumi, Kosuke Terushima, Yousuke Katsuda, Kumi Hidaka, Yoshie Harada, and Hiroshi Sugiyama Direct Visualization of the Movement of a Single T7 RNA Polymerase and Transcription on a DNA Nanostructure
  • Jinglin Fu, Minghui Liu, Yan Liu, Neal W. Woodbury, and Hao Yan Interenzyme Substrate Diffusion for an Enzyme Cascade Organized on Spatially Addressable DNA Nanostructures

15th

the fourth meeting

We read in turn following two papers.

  • Souvik Modi, Swetha M. G., Debanjan Goswami, Gagan D. Gupta, Satyajit Mayor and Yamuna Krishnan A DNA nanomachine that maps spatial and temporal pH changes inside living cells
  • Masayuki Endo, Ryoji Miyazaki, Tomoko Emura, Kumi Hidaka, and Hiroshi Sugiyama Transcription Regulation System Mediated by Mechanical Operation of a DNA Nanostructure

22th

the fifth meeting

We read in turn following two papers.

  • Shawn M. Douglas, et al. A Logic-Gated Nanorobot for Targeted Transport of Molecular Payloads
  • Xibo Shen, Qiao Jiang, Jinye Wang, Luru Dai, Guozhang Zou, Zhen-Gang Wang, Wei-Qiang Chen, Wei Jiang and Baoquan Ding Visualization of the intracellular location and stability of DNA origami with a label-free fluorescent probe

29th

June

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