Cong T. Trinh:media
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Acetobactor aceti
YPD (Uwe sauer)
Chemicals Concentration glucose 30 g/L bacto peptone 2 g/L yeast extract 5 g/L
- Grow at 30°C
ATCC medium
Chemicals Concentration yeast extract 5 g/L peptone 3 g/L mannitol 25 g/L
E. coli
2 LB++
Chemicals Concentration 2xLB -see below MgSO4 2 g/L NH4SO4 1 g/L Glucose 20 g/L
910 Broth
Chemicals Concentration (NH4)2HPO4 10 g/L K2HPO4 5 g/L Na2SO4 0.5 g/L Octane 1% (v/v)
B00BIES
Chemicals Concentration Tryptone 10 g/L Yeast Extract 5 g/L 5x M9 Salts 200 mL/L 1M MgSO4 2 mL/L 1M CaCl2 0.1 mL/L
LB(Low Salt/ general use)
Chemicals Concentration Tryptone 10 g/L Yeast Extract 5 g/L NaCl 5 g/L
LB(plates)
Chemicals Concentration Tryptone 10 g/L Yeast Extract 5 g/L NaCl 5 g/L Agar 20 g/L
M9 Minimal Media
Chemicals Concentration Make M9 salts To make M9 Salts aliquot 800ml H2O and add 64g Na2HPO4-7H2O 15g KH2PO4 2.5g NaCl 5.0g NH4Cl Stir until dissolved Adjust to 1000ml with distilled H2O Sterilize by autoclaving Measure ~700ml of distilled H2O (sterile) Add 200ml of M9 salts Add 2ml of 1M MgSO4 (sterile) Add 20 ml of 20% glucose (or other carbon source) Add 100ul of 1M CaCl2 (sterile) Adjust to 1000ml with distilled H2O
Minimal buffer A for random mutagenesis
Chemicals Concentration K2HPO4 10.5 g/L KH2PO4 4.5 g/L (NH4)2SO4 1.0 g/L NaCIT.2H2O 0.5 g/L
SOB Media
Chemicals Concentration Tryptone 20 g/L Yeast extract 5 g/L NaCl 0.5 g/L KCl 0.2 g/L MgSO4 2.4 g/L pH 7
SOC MEdia
Same as SOB + 20mM Glucose
Stock trace metals solution
Chemicals Concentration H3BO4 0.15 g/L CoSO4 0.065 g/L ZnSO4.7H2O 0.05 g/L MnCl2.4H2O 0.015 g/L NaMo4.2H2O 0.015 g/L NiCl2.6H2O 0.01 g/L CuSO4.5H2O 0.005 g/L Fe(NH4)Citrate 3 g/L
Z broth
Chemicals Concentration LB 50mL CaCl2 25μL of 1M CaCl2
Antibiotic Working Concentration
Antibiotic Concentration Ampicillin 100 μg/mL Kanamycin 50 μg/mL Chloramphenicol 25 μg/mL Tetracycline 50 μg/mL Amp+Kan 25 μg/mL of each
P. putida
Normal growth media
Chemicals Concentration LB glucose 20 g/L
- Grow at 30°C
Z. mobilis
ATCC medium
Chemicals Concentration yeast extract 10 g/L glucose 20 g/L KH2PO4 2 g/L
- adjust pH to 6.5
PL Rogers
Chemicals Concentration yeast extract 10 g/L glucose 20 g/L MgSO4.7H2O 1 g/L KH2PO4 2 g/L (NH4)2SO4 1 g/L
