Carrico:Buffers

Carrico Lab at Stony Brook

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DNA Gel Electrophoresis

50x TAE Buffer (to make 1L)

• 242g 2M Tris base
• 57.1mL 1M glacial acetic acid
• 200mL 100mM EDTA
• Add water up to 1L

Note: To dilute 50x to 1x, add 20mL of 50x to 980mL water