Calendar:ImperialWetLabNotebook2009iGEM/2009-9-1

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AM

- SLIC Primer design

- PCV FMA assay

PM

- Transforming biobricks into cells & plating

- Miniprep prep for the three Edinburgh cellulases


AM

- Restriction digest on I13504, B0034 (1), B0034 (2), K118011 (1), K118011 (2), R0011, K098995

- Gel extraction on GFP (I13504) from last week (Placed in freezer labelled GRP GE 1/09/09)

PM

- Run K118011 (2) on a 1.5% gel and cut out gel (give to Jim)

- Dephosphorylation for dirty ligations for RBS (B0034) + AK3

- Ligation for RBS (B0034) + AK3 (placed in float in water bath in cold room)

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