CTR:Notebook/Migrant/2016/11/14

Project name

Main project page Previous entry      Next entry

SWTH Rhodopsin Amplified DNA PCR done by Uma 11/8/2016, Will Richardson Samples + Dan Inserillo Samples Band in the PCR -ve well indicates DNA contamination PCR done by Uma 11/14/2016, Will Richardson Samples Test for contamination. First 4 samples run using old reagents used in previous PCR. Second 4 samples, changed water, primer mix, and Qiagen Master Mix Contamination persists indicated by band in the PCR -ve well PCR done by Winnie 11/15/2016, Will Richardson Samples Test for contamination All new reagents used Contamination persists indicated by band in the PCR -ve well PCR done by Winnie and Uma 11/16/2016, Will Richardson Samples Test for contamination Well 2 and 3 represent the repeated comparison of old reagents vs new reagents PCR -ves only, keeping the Qiagen Master Mix the same Well 5 - 9 represent PCR done with reagents from the Pre-PCR room and with the Pre-PCR room pipettes PCR preparation in the lab (Pre-PCR bench) still indicate contamination, however PCR preparation in the Pre-PCR room shows no contamination However, extract negative shows a band, so there is still the possibility of contamination during DNA extraction