Bitan:qpcr
From OpenWetWare
Jump to navigationJump to search
Sample Protocol
| Cycle | Repeats | Step | Dwell Time | Setpoint | Melt Curve | Temp+ | Temp- |
| 1 | 1 | 1 | 5:00 | 95 | |||
| 2 | 50 | 1 | 0:30 | 95 | |||
| 2 | 0:30 | 65 | |||||
| 3 | 0:30 | 72 | |||||
| 3 | 1 | 1 | 1:00 | 95 | |||
| 4 | 1 | 1 | 1:00 | 55 | |||
| 5 | 80 | 1 | 0:10 | 55 | x | 0.5 |
