Biomod/2013/HKBU/approaches

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BIOMOD 2013 The Rising Power

More Microscopic Imaging

Click herefor more FM imaging.

Click herefor more AFM imaging.

Equipment

Bio-Rad ChemiDoc XRS

Purpose: Perform UV-illumination and visualize the agarose gel after electrophoresis

Bio-Rad PowerPac™ Basic Power Supply and Bio-Rad PowerPac™ HC High-Current Power Supply

Purpose: Provide constant voltage for gel electrophoresis

Eppendorf Centrifuge 5417C

Purpose: Conduct gel extraction

UVP C-80 Epi- Illumination Darkroom

Purpose: Perform excision of targeted bands on the agarose gel

QIAquick Gel Extraction Kit (QIAGEN, USA)

BIOMOD Team Member

Purpose: Perform gel extraction

NucleoSpin® Extract II Kits (Macherey-Nagel, Germany)

Purpose: Perform gel extraction

BioScope Catalyst Atomic Force Microscope (Bruker Nano, Santa Barbara, CA, U.S.A.)

Purpose: conduct atomic force microscopy

LEO 1530 Field Emission SEM (Carl Zeiss, Germany)

Purpose: conduct scanning electron microscopy for quantum dots

Inverted Olympus IX-71 Microscope (Olympus, Tokyo, Japan)

Purpose: conduct total internal reflection fluorescence microscopy

Applied Biosystems® Veriti® 96-Well Thermal Cycler

Purpose: Perform annealing for self-assembly of DNA origami

NanoDrop ND-1000 Spectrophotometer

Purpose: Determine the concentration and relative purity of DNA

Eppendorf Centrifuge 5430R

Purpose: Conduct DNA purification in low-temperature condition


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