Biomod/2013/Columbia

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Enzymers: The polymerization of functional enzyme chains

Abstract: Enzymes are the catalysts to every chemical reaction that allows for the existence of life. In nature, many chemical reactions are coupled, with the product of one reaction being used as the reagent for another nearby enzyme. The aim of this project is to couple Glucose Oxidase and Horseradish Peroxidase, an enzyme pair used in spectrophotometer assays, using azide-alkyne click chemistry via NHS ester conjugation. Increased efficiency and yield of a coupled reaction would have tremendous impact on the field of chemical creation and chemical purchases.

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